Instead, Samson Siasia’s charges will take on an unnamed team in the United States of America on the same date.
The Nigerian team’s media officer Timi Ebikagboro revealed on Twitter on Thursday that the friendly would no longer go ahead.
No reason was attributed for the cancellation of the friendly.
The U-23 Eagles beat Charleston Battery FC 1-0 in their last friendly game on Sunday with left-back Stanley Amuzie scoring the winner.
Samson Siasia has released a list of 22 players that will play in the football event of the Rio 2016 Olympic Games.
The 1996 Olympic gold medallists will play Sweden, Japan and Colombia in the group stage of the event.